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Health

Serological tests

Pertussis: antibodies to Bordetella pertussis in serum

In recent years, test systems have been developed that make it possible to determine antibodies of classes IgA, IgM and IgG to Bordetella pertussis antigens in serum by ELISA. IgM antibodies appear in the blood at the 3rd week from the onset of the disease, so they can be used to confirm the etiologic diagnosis. The dynamics of IgA antibody titer to Bordetella pertussis toxin is similar to that of IgM.

Diphtheria: antibodies to diphtheria toxin in the blood

Serological methods for the diagnosis of diphtheria use indirect hemagglutination and ELISA. Determine the antibody titre to the diphtheria toxin at the onset of the disease (day 1-3) and after 7-10 days, the antibody titre growth is considered to be diagnostic by no less than 4 times.

Tuberculosis: antibodies to the causative agent of tuberculosis in the blood

Determination of antibodies to the causative agent of tuberculosis in serum is a new and very promising method for the serological diagnosis of tuberculosis. The currently used bacteriological method for isolating the mycobacterium of tuberculosis requires considerable time-consuming (4 to 8 weeks) and is very effective mainly in the pulmonary forms of tuberculosis.

Salmonellosis: antibodies to salmonella in the blood

Currently, to identify antibodies to Salmonella (to O-antigen), the most widely used are RPGA and ELISA, they are more sensitive than Vidal's and give positive results from the 5th day of the disease (Vidal's reaction on the 7th-8th day ).

Brucellosis: antibodies to the causative agent of brucellosis in the blood

The causative agents of brucellosis are brucellae, small immobile gram-negative bacteria. When diagnosing brucellosis, the obtained clinical and epidemiological data should be confirmed laboratory.

Determination of Neisseria meningitis antigens in cerebrospinal fluid

Of great importance for the early diagnosis of meningococcal infection is a study of cerebrospinal fluid in patients with meningeal symptoms for the detection of Neisseria meningitis antigens.

Antibodies to meningococcus in serum

The detection of antibodies to meningococcus is used to diagnose meningococcal infection in bacterial and serous meningitis, as well as with urethritis.

Antibodies to hemophilic rod in serum

For serological diagnosis of diseases caused by stick influenza, agglutination and precipitation reactions are used. Determination of antibodies to hemophilic rod in serum is a retrospective method of diagnosing the disease, since it is necessary to examine serum in the first week of the disease and after 10-14 days.

Antibodies to pneumococcus in serum

Serological diagnosis of pneumococcal infection is aimed at detecting the titer of anticapsular antibodies in the patient's blood serum. The increase in the antibody titer is considered to be diagnostic after 7-10 days for the study of paired sera.

Staphylococcal infections: antibodies to staphylococci in serum

From the serological methods of diagnosis of purulent-septic diseases apply the reaction of direct hemagglutination and ELISA. The increase in the antibody titer is considered to be diagnostic after 7-10 days for the study of paired sera.

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