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Aggregation of platelets with collagen
Last reviewed: 23.04.2024
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Collagen-induced platelet aggregation has a fairly pronounced latent phase, during which phospholipase C is activated. Depending on the reagent used, the duration of this phase can be 5-7 minutes. After the end of this period, platelet-forming processes lead to the formation of secondary mediators, resulting in secretion of platelet granules and synthesis of thromboxane A 2, which is accompanied by a sharp increase in interthrombocyte interaction.
In laboratory and clinical practice, collagen is most often used at a final concentration of 50 μg / ml, but collagens from different firms may have different activities, which must be taken into account when using them. The results of the study of platelet aggregation capacity can be expressed as a percentage.
Separately, this study is not used, but carried out in conjunction with the determination of platelet aggregation with ADP and adrenaline.
Reference values of platelet aggregation according to Weiss for collagen
|
Concentration of collagen, μg / ml |
Aggregation is normal,% |
|
10 |
93.1 |
|
5 |
75.0 |
|
2 |
69.4 |
|
1 |
46.4 |
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