Express diagnosis of urogenital chlamydiosis
Last reviewed: 23.04.2024
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Chlamydia trachomatis in the material from the urogenital organs is not normally present.
The method is based on the detection of Chlamydia trachomatis antigens in scrapings from the urethra, cervical canal and conjunctiva by ELISA with a visual evaluation of the result (sensitivity is more than 79%, specificity is more than 95%). This method is based on the presence in chlamydia of the rhodospecific lipopolysaccharide antigen. It allows rapid screening of the pathogen, but the final diagnosis is established using the fluorescent antibody method or PCR. The results of the study are expressed in the form of a positive or negative answer. To obtain satisfactory results of the study, it is necessary to follow certain rules: the material (scraping) should be correctly taken and delivered to the laboratory in a timely manner (within 2 hours).
Express diagnosis of urogenital chlamydiosis is used for urethritis, prostatitis, cervicitis, adnexitis.
[Determination of Chlamydia trachomatis by the method of fluorescent antibodies
Chlamydia trachomatis in the material from the urogenital organs is not normally present.
The principle of the method is the use of monoclonal antibodies labeled with a fluorescent isothiocyanate against the main protein of the outer membrane of chlamydia present in all the Chlamydia trachomatis serovars , as well as in the elementary and reticular bodies. The sensitivity of the method reaches 90-95%, and the use of monoclonal antibodies causes high specificity - more than 95% in the presence of clinical manifestations of urogenital chlamydia.
In the study of scrapings from the conjunctiva, the sensitivity of the fluorescent antibody method is 70-95%, specificity 98%.