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Rotavirus infection - Diagnosis
Last reviewed: 03.07.2025
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Diagnosis of rotavirus infection is based on clinical and diagnostic signs of rotavirus infection:
- characteristic epidemiological history - group nature of the disease in the winter season;
- acute onset of the disease;
- increased body temperature and intoxication syndrome;
- vomiting as a leading symptom;
- watery diarrhea;
- moderate abdominal pain; o flatulence.
Laboratory diagnostics of rotavirus infection uses three groups of methods:
- methods based on the detection of rotavirus and its antigens in feces:
- electron and immunoelectron microscopy;
- RLA$:
- IFA;
- methods for detecting viral RNA in coprofiltrates:
- molecular probe method - PCR and hybridization;
- RNA electrophoresis in polyacrylamide gel or agarose;
- methods for detecting specific antibodies (immunoglobulins of various classes and/or increasing antibody titers) to rotaviruses in blood serum (ELISA, RSC, RTGA, RIGA).
In practice, the diagnosis of rotavirus infection is most often based on the detection of viral antigen in coprofiltrates using RLA and ELISA on the 1st to 4th day of illness.
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