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Rotavirus infection: diagnosis
Last reviewed: 23.04.2024
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Diagnosis of rotavirus infection is based on clinical and diagnostic signs of rotavirus infection:
- characteristic epidemiological history - the group nature of the disease in the winter season;
- acute onset of the disease;
- increased body temperature and intoxication syndrome;
- vomiting as a leading symptom;
- watery diarrhea;
- moderate pain in the abdomen; o flatulence.
Laboratory diagnostics of rotavirus infection uses three groups of methods:
- methods based on the detection of rotavirus and its antigens in faeces:
- electronic and immunoelectronic microscopy;
- RLA $:
- EIA;
- methods for detecting viral RNA in coprofiltrates:
- method of molecular probes - PCR and hybridization;
- RNA electrophoresis in polyacrylamide gel or agarose;
- methods for the detection of specific antibodies (immunoglobulins of different classes and / or buildup of antibody titer) to rotaviruses in serum (ELISA, RSA, RTGA, RIGA).
In practice, the diagnosis of rotavirus infection is most often based on the detection of viral antigen in coprofiltrates using RLA, ELISA in the 1-4 days of the disease.
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